| jetclhp7 | Date: Monday, 09.23.2013, 1:08 PM | Message # 1 |
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| 3. Keep in mind various carryon size restrictions by airline. Both personal bags and carryon bags are subject to size (and in some cases, weight) restrictions. It is important to note that restrictions for personal bags are different than those for carryon bags. Moreover, restrictions also vary by airline. Unfortunately, this means that one carryon bag may not work for all airlines. Make sure you look up the size and weight limitations for the airline you are flying in advance. Here is a carryon luggage size chart that breaks down each airline restrictions by personal bag and carryon bag.
influence of freezing parameters on cell recovery and engraftment aCorrespondence: F Beaujean, Laboratoire de Th Cellulaire, Etablissement de Transfusion Sanguine du SudFrancilien, H Henri Mondor, 51, Avenue du Mar de Lattre de Tassigny, 94010 Cr FranceConventional hematopoietic stem cell cryopreservation methods use a DMSO concentration of 10%. However, cells manipulated ex vivo may require more refined freezing protocols adapted to the specific cell suspension. In this retrospective study, we evaluated the results obtained with CD34+ cells purified from peripheral blood of 39 patients on the CEPRATE SC System and frozen in 7.5% DMSO with a view to transplantation. The postfreezing recovery of progenitor cells was 89.4 27.87% for CD34+ cells, 59.13 36.93% for CFUGM, and 53.49 40.71 for BFUE. Neither the purity of the suspension nor the nucleated cell density during freezing was predictive of cell recovery. No difference was observed between cells stored in vials and bags. Thirtyseven patients transplanted with the concentrated CD34+ fraction received 4.46 106 CD34+ cells/kg and 33.04 104 CFUGM/kg. The median time to granulocyte (>0.5 109/l) and platelet (>50 109/l) engraftment was 11 and 13 days, respectively. Only cell density and the infused number of CD34+ cells and CFUGM were significantly related to hematological recovery. Our data suggest that purified CD34+ cells can be successfully cryopreserved in 7.5% DMSO and may represent a first step in establishing freezing parameters for selected CD34+ cells.
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